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Anti-complement C5a Antibody

     
  • WB - Anti-complement C5a Antibody ABO11617
    Anti-C5/C5a antibody, ABO11617, All Western blottingAll lanes: Anti-C5a(ABO11617) at 0.5ug/mlWB: Rat Liver Tissue Lysate at 40ugPredicted bind size: 115KDObserved bind size: 115KD
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P08650
Host Rabbit
Reactivity Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Complement C5(C5) detection. Tested with WB in Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Other Names Complement C5, C5a anaphylatoxin, C5
Calculated MW 8981 MW KDa
Application Details Western blot, 0.1-0.5 µg/ml, Rat
Subcellular Localization Secreted.
Protein Name Complement C5
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of rat C5a(59-77aa TIADKIRKESHHKGMLLGR), different from the related mouse sequence by five amino acids.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name C5
Function Derived from proteolytic degradation of complement C5, C5 anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
Cellular Location Secreted. EMBL; X91892; CAA62994.1; -; mRNA PIR; A57689; A57689 UniGene; Rn.21259; - ProteinModelPortal; P08650; - SMR; P08650; - STRING; 10116.ENSRNOP00000025534; - PaxDb; P08650; - PRIDE; P08650; - UCSC; RGD:2237; rat RGD; 2237; C5 eggNOG; ENOG410IMYH; Eukaryota eggNOG; ENOG410ZFKB; LUCA InParanoid; P08650; - Proteomes; UP000002494; Unplaced GO; GO:0005623; C:cell; IEA:GOC GO; GO:0005615; C:extracellular space; IDA:RGD GO; GO:0031714; F:C5a anaphylatoxin chemotactic receptor binding; IDA:RGD GO; GO:0006874; P:cellular calcium ion homeostasis; IDA:RGD GO; GO:0006935; P:chemotaxis; IDA:RGD GO; GO:0006957; P:complement activation, alternative pathway; IEA:UniProtKB-KW GO; GO:0006958; P:complement activation, classical pathway; IEA:UniProtKB-KW GO; GO:0042593; P:glucose homeostasis; IDA:RGD GO; GO:0006954; P:inflammatory response; IDA:RGD GO; GO:0002523; P:leukocyte migration involved in inflammatory response; IMP:RGD GO; GO:0033602; P:negative regulation of dopamine secretion; IDA:RGD GO; GO:0010700; P:negative regulation of norepinephrine secretion; IDA:RGD GO; GO:0050921; P:positive regulation of chemotaxis; IDA:RGD GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; IDA:RGD InterPro; IPR000020; Anaphylatoxin/fibulin InterPro; IPR018081; Anaphylatoxin_comp_syst InterPro; IPR001840; Anaphylatoxn_comp_syst_dom InterPro; IPR037562; Complement_C5 PANTHER; PTHR11412:SF83; PTHR11412:SF83; 1 Pfam; PF01821; ANATO; 1 PRINTS; PR00004; ANAPHYLATOXN SMART; SM00104; ANATO; 1 SUPFAM; SSF47686; SSF47686; 1 PROSITE; PS01177; ANAPHYLATOXIN_1; 1 PROSITE; PS01178; ANAPHYLATOXIN_2; 1 1: Evidence at protein level; Complement alternate pathway; Complement pathway; Complete proteome; Direct protein sequencing; Disulfide bond; Immunity; Inflammatory response; Innate immunity; Reference proteome; Secreted CHAIN 1 77 C5a anaphylatoxin /FTId=PRO_0000005996 DOMAIN 24 58 Anaphylatoxin-like. {ECO:0000255|PROSITE- ProRule:PRU00022} REGION 18 47 Involved in C5AR1 binding {ECO:0000250|UniProtKB:P01031} DISULFID 24 50 {ECO:0000255|PROSITE-ProRule:PRU00022} DISULFID 25 57 {ECO:0000255|PROSITE-ProRule:PRU00022} DISULFID 37 58 {ECO:0000255|PROSITE-ProRule:PRU00022} CONFLICT 55 55 N -> K (in Ref. 1; AA sequence and 2; AA sequence). CONFLICT 61 63 ADK -> DP (in Ref. 1; AA sequence) CONFLICT 63 63 K -> H (in Ref. 2; AA sequence) CONFLICT 67 69 ESH -> NES (in Ref. 2; AA sequence) CONFLICT 67 69 ESH -> NQS (in Ref. 1; AA sequence) NON_TER 1 1 NON_TER 77 77 SEQUENCE 77 AA; 8981 MW; 14141F41CC38BD28 CRC64; DLQLLHQKVE EQAAKYKHRV PKKCCYDGAR ENKYETCEQR VARVTIGPHC IRAFNECCTI ADKIRKESHH KGMLLGR
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Background

C5a is a protein fragment released from complement component C5. This gene is mapped to 9q33.2. The protein encoded by this gene is the fifth component of complement, which plays an important role in inflammatory and cell killing processes. This protein is comprised of alpha and beta polypeptide chains that are linked by a disulfide bridge. An activation peptide, C5a, which is an anaphylatoxin that possesses potent spasmogenic and chemotactic activity, is derived from the alpha polypeptide via cleavage with a convertase. The C5b macromolecular cleavage product can form a complex with the C6 complement component, and this complex is the basis for formation of the membrane attack complex, which includes additional complement components. Mutations in this gene cause complement component 5 deficiency, a disease where patients show a propensity for severe recurrent infections. Defects in this gene have also been linked to susceptibility to liver fibrosis and to rheumatoid arthritis.

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$ 240.00
Cat# ABO11617
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