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Anti-Wnt1 Picoband Antibody

     
  • WB - Anti-Wnt1 Picoband Antibody ABO12149
    Anti- WNT1 Picoband antibody, ABO12149, Western blottingAll lanes: Anti WNT1 (ABO12149) at 0.5ug/mlWB: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 41KDObserved bind size: 41KD
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB
Primary Accession P04628
Host Rabbit
Reactivity Human
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Proto-oncogene Wnt-1(WNT1) detection. Tested with WB in Human.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 7471
Other Names Proto-oncogene Wnt-1, Proto-oncogene Int-1 homolog, WNT1, INT1
Calculated MW 40982 MW KDa
Application Details Western blot, 0.1-0.5 µg/ml, Human
Subcellular Localization Secreted, extracellular space, extracellular matrix.
Protein Name Proto-oncogene Wnt-1
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human Wnt1 (45-74aa NLLTDSKSLQLVLEPSLQLLSRKQRRLIRQ), identical to the related mouse and rat sequences.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Sequence Similarities Belongs to the Wnt family.
Protein Information
Name WNT1
Synonyms INT1
Function Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Acts in the canonical Wnt signaling pathway by promoting beta-catenin-dependent transcriptional activation (PubMed:23499309, PubMed:26902720, PubMed:28528193, PubMed:23656646). In some developmental processes, is also a ligand for the coreceptor RYK, thus triggering Wnt signaling (By similarity). Plays an essential role in the development of the embryonic brain and central nervous system (CNS) (By similarity). Has a role in osteoblast function, bone development and bone homeostasis (PubMed:23499309, PubMed:23656646).
Cellular Location Secreted, extracellular space, extracellular matrix. Secreted
Research Areas
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Background

Wingless-type MMIV integration site family, member 1 is a protein that in humans is encoded by the WNT1 gene. This gene is a member of the WNT gene family. The gene was assigned to human chromosome 12q13.12. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. The Wnt1 protein functions in the induction of the mesencephalon and cerebellum. However, the gene mutations might not have a significant role in Joubert syndrome.

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$ 240.00
Cat# ABO12149
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