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Anti-TRIB2 Picoband Antibody

     
  • WB - Anti-TRIB2 Picoband Antibody ABO12586
    Western blot analysis of TRIB2 expression in SW620 whole cell lysates (lane 1). TRIB2 at 39KD was detected using rabbit anti- TRIB2 Antigen Affinity purified polyclonal antibody (Catalog # ABO12586) at0.5 ??g/mL. The blot was developed using chemiluminescence (ECL) method .
  • IHC - Anti-TRIB2 Picoband Antibody ABO12586
    TRIB2 was detected in paraffin-embedded sections of mouse spleen tissues using rabbit anti- TRIB2 Antigen Affinity purified polyclonal antibody (Catalog # ABO12586) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
  • IHC - Anti-TRIB2 Picoband Antibody ABO12586
    TRIB2 was detected in paraffin-embedded sections of rat spleen tissues using rabbit anti- TRIB2 Antigen Affinity purified polyclonal antibody (Catalog # ABO12586) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
  • IHC - Anti-TRIB2 Picoband Antibody ABO12586
    TRIB2 was detected in paraffin-embedded sections of human tonsil tissues using rabbit anti- TRIB2 Antigen Affinity purified polyclonal antibody (Catalog # ABO12586) at 1 μg/mL. The immunohistochemical section was developed using SABC method .
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC-P
Primary Accession Q92519
Host Rabbit
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Format Lyophilized
Description Rabbit IgG polyclonal antibody for Tribbles homolog 2(TRIB2) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Additional Information
Gene ID 28951
Other Names Tribbles homolog 2, TRB-2, TRIB2 (HGNC:30809)
Calculated MW 38801 MW KDa
Application Details Immunohistochemistry(Paraffin-embedded Section), 0.5-1 µg/ml, Human, Mouse, Rat, By Heat

Western blot, 0.1-0.5 µg/ml, Human
Subcellular Localization Cytoplasm . Cytoplasm, cytoskeleton . May associate with the cytoskeleton. .
Tissue Specificity Highly expressed in peripheral blood leukocytes. .
Protein Name Tribbles homolog 2
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human TRIB2 (175-211aa DLKLRKFIFKDEERTRVKLESLEDAYILRGDDDSLSD), identical to the related mouse sequence.
Purification Immunogen affinity purified.
Cross Reactivity No cross reactivity with other proteins.
Storage At -20˚C for one year. After r˚Constitution, at 4˚C for one month. It˚Can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Protein Information
Name TRIB2 (HGNC:30809)
Function Interacts with MAPK kinases and regulates activation of MAP kinases. Does not display kinase activity (By similarity).
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton. Note=May associate with the cytoskeleton.
Tissue Location Highly expressed in peripheral blood leukocytes.
Research Areas
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Background

Tribbles homolog 2 is a protein that in humans is encoded by the TRIB2 gene. This gene encodes one of three members of the Tribbles family. The Tribbles members share a Trb domain, which is homologous to protein serine-threonine kinases, but lacks the active site lysine and probably lacks a catalytic function. The Tribbles proteins interact and modulate the activity of signal transduction pathways in a number of physiological and pathological processes. This Tribbles member induces apoptosis of cells mainly of the hematopoietic origin. It has been identified as a protein up-regulated by inflammatory stimuli in myeloid (THP-1) cells, and also as an oncogene that inactivates the transcription factor C/EBP alpha (CCAAT/enhancer-binding protein alpha) and causes acute myelogenous leukemia. Alternatively spliced transcript variants have been found for this gene.

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$ 280.00
Cat# ABO12586
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