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Caspase-3 Antibody (Clone C33)

Mouse Monoclonal Antibody

  • WB - Caspase-3 Antibody (Clone C33) ABV10002
    Western blot analysis of caspase-3 expression in HeLa cells treated with camptothecin (2 μM).
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P42574
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Isotype Mouse IgG1
Clone Names C33
Calculated MW 31608 Da
Additional Information
Gene ID 836
Application & Usage Western blotting (0.5-4 µg/ml). The antibody recognizes both proform and the cleaved large fragment of caspase-3 in samples of human, mouse and rat origins. However, the optimal conditions should be determined individually.
Other Names CPP32 , CASP3, apopain, procaspase3, CPP32B, SCA-1, CPP-32, Apopain, Yama
Target/Specificity Caspase-3
Antibody Form Liquid
Appearance Colorless liquid
Formulation 100 µg (200 µg/ml) in PBS containing 50% glycerol, 0.5 mg/ml BSA and 0.01% thimerosal.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsCaspase-3 Antibody (Clone C33) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CASP3
Synonyms CPP32
Function Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage.
Cellular Location Cytoplasm.
Tissue Location Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system
Research Areas
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The caspase family of cysteine proteases play a key role in apoptosis. Caspase-3 is the most extensively studied apoptotic protein among caspase family members. Caspase-3 is synthesized as inactive pro-enzyme that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-3 consists of large (17 kD) and small (12 kD) subunits which associate to form an active enzyme. The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP and DFF).

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$ 295.00
Cat# ABV10002
(40 western blots)
Availability: 5-7days
Bulk Size
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