|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||50661 Da|
|Positive Control||Western Blot: Jurkat cell lysate, treated and untreated. IHC: Placenta tissue.|
|Application & Usage||Western blotting (1-2 µg/ml), and Immunohistochemistry (5 µg/ml). However, the optimal conditions should be determined individually. Detects the full length and small subunit of caspase-2. In human samples, it also detects a 30 kDa intermeadiate fragment|
|Other Names||CASP-2, NEDD-2, NEDD2, ICH1, ICH-1L/1S, ICH-1L, EC 18.104.22.168|
|Formulation||100 µg (0.2 mg/ml) affinity purified rabbit anti-caspase-2 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Caspase-2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||Ich1, Nedd-2, Nedd2|
|Function||Involved in the activation cascade of caspases responsible for apoptosis execution. Might function by either activating some proteins required for cell death or inactivating proteins necessary for cell survival. May be important in multistep carcinogenesis.|
|Tissue Location||High level expression seen in the embryonic CNS, liver, lung, kidney, small intestine, and hair follicles of vibrissae. Moderate expression seen in the skin, oral mucosa, skeletal muscle, submandibular gland and thymus. In the adult, it is highly expressed in spleen, lung and kidney. Moderately in the brain, heart, testis, liver. Low levels in the thymus, skeletal muscle, ovary and gut|
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Provided below are standard protocols that you may find useful for product applications.
Caspases play a key role in apoptosis. Similar as other caspases, caspase-2 is also synthesized as an inactive pro-enzyme that is processed in cells undergoing apoptosis. Caspase-2 mRNA is alternatively spliced. The larger mRNA species encoding a product of 435 amino acids is known as ICH-1L. The smaller mRNA species encoding a protein of 312 amino acids is named ICH-1S. Over expression of ICH-1L induces apoptosis, while over expression of ICH-1S suppresses Rat-1 cell death induced by serum deprivation.
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