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BAK1 Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | Q16611 |
| Reactivity | Human |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 23409 Da |
| Gene ID | 578 |
|---|---|
| Positive Control | Jurkat cell lysate |
| Application & Usage | Western blot analysis (1-4 µg/ml) and immunoprecipitation (5-20 µg/ml). |
| Other Names | Apoptosis regulator BAK, Bcl-2-like protein 7 |
| Target/Specificity | Bak1 |
| Antibody Form | Liquid |
| Appearance | Colorless liquid |
| Formulation | 100 µg (0.5 mg/ml) peptide affinity purified rabbit anti-Bak polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal. |
| Handling | The antibody solution should be gently mixed before use. |
| Reconstitution & Storage | -20 °C |
| Background Descriptions | |
| Precautions | BAK1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | BAK1 |
|---|---|
| Synonyms | BAK, BCL2L7, CDN1 |
| Function | Plays a role in the mitochondrial apoptotic process. Upon arrival of cell death signals, promotes mitochondrial outer membrane (MOM) permeabilization by oligomerizing to form pores within the MOM. This releases apoptogenic factors into the cytosol, including cytochrome c, promoting the activation of caspase 9 which in turn processes and activates the effector caspases. |
| Cellular Location | Mitochondrion outer membrane; Single-pass membrane protein |
| Tissue Location | Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
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Provided below are standard protocols that you may find useful for product applications.
Background
Bak is a homolog of apoptosis inhibitor Bcl-2. Bak is a member of the Bcl-2 family of proteins and contains Bcl-2 homology region (BH-) 1, 2 and 3. Unlike Bcl-2, Bak promotes cell death and counteracts the anti-apoptotic effect of Bcl-2. The immunoaffinity purified antibody detects a 30 kDa band, corresponding to the expected molecular weight of Bak on SDS-PAGE immunoblots.
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