|Calculated MW||23409 Da|
|Positive Control||Jurkat cell lysate|
|Application & Usage||Western blot analysis (1-4 µg/ml) and immunoprecipitation (5-20 µg/ml).|
|Other Names||Apoptosis regulator BAK, Bcl-2-like protein 7|
|Formulation||100 µg (0.5 mg/ml) peptide affinity purified rabbit anti-Bak polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||BAK1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||BAK, BCL2L7, CDN1|
|Function||In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti- apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis.|
|Cellular Location||Mitochondrion membrane; Single-pass membrane protein|
|Tissue Location||Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle|
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Provided below are standard protocols that you may find useful for product applications.
Bak is a homolog of apoptosis inhibitor Bcl-2. Bak is a member of the Bcl-2 family of proteins and contains Bcl-2 homology region (BH-) 1, 2 and 3. Unlike Bcl-2, Bak promotes cell death and counteracts the anti-apoptotic effect of Bcl-2. The immunoaffinity purified antibody detects a 30 kDa band, corresponding to the expected molecular weight of Bak on SDS-PAGE immunoblots.
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