|Application ||WB, IHC, IP|
|Reactivity||Human, Mouse, Rat, Monkey|
|Calculated MW||37337 Da|
|Application & Usage||Western blot analysis (0.5-4 µg/ml), immunoprecipitation (10-20 µg/ml), and Immunohistochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually.|
|Other Names||MCL1L, mcl1/EAT, MGC1839, MGC104264, TM, MCL1S, EAT|
|Formulation||100 µg (0.2 mg/ml) affinity purified rabbit anti-Mcl-1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Mcl-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in the regulation of apoptosis versus cell survival, and in the maintenance of viability but not of proliferation. Mediates its effects by interactions with a number of other regulators of apoptosis. Isoform 1 inhibits apoptosis. Isoform 2 promotes apoptosis.|
|Cellular Location||Membrane; Single-pass membrane protein. Cytoplasm. Mitochondrion. Nucleus, nucleoplasm. Note=Cytoplasmic, associated with mitochondria|
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Mcl-1 (Myeloid cell leukemia-1) is a 37 kDa Bcl-2-related protein with an apparent molecular weight of 43 kDa on SDS-PAGE. Mcl-1 was identified as an early-induction gene that increased in expression during the differentiation of human myeloblastic leukemia cell ML-1, or exposure to different DNA damaging agents. The level of Mcl-1 is decreased in peripheral B lymphocytes undergoing apoptosis following treatment with apoptotic stimuli such as TGF-β1 and forskolin. Expression of Mcl-1 is able to delay apoptosis induced by over-expression of c-myc in CHO 5AHSmyc cells. In hematopoietic FDC-P1 cells, Mcl-1 interacts with another Bcl-2-related protein, Bax, and prolongs cell viability after treatment with different apoptotic reagents.
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