|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Application & Usage||Western blot analysis (0.5-4 µg/ml) and Immunohistochemistry (5 µg/ml). However, the optimal conditions should be determined individually.|
|Other Names||WAL-1, APO-3, TRAMP, LARD|
|Formulation||100 µg (0.5 mg/ml) antigen affinity-purified rabbit anti-DR3/Apo3 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||DR3/Apo3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Apoptosis can be induced by certain cytokines including TNF and Fas in the TNF superfamily thro µgh interaction with their death domain (DD) containing receptors, TNFR1 and Fas. A member of the TNF receptor family was recently identified and designated DR3 (also called Wal-1, Apo3, TRAMP and LARD). The ligand for this novel death receptor has been defined as TWEAK (also termed Apo3L). Like TNFR1, overexpression of DR3 triggers apoptosis and NF-kB activation. Activation of DR3 results in recruitment of TRADD (a DD containing adapter molecule). TRADD associates with FADD, TRAF2, and RIP to activate the signaling complex for NF-kB activation. DR3 activates apoptosis thro µgh TRADD, FADD, and caspase-8. DR3 is highly expressed in the tissues enriched in lymphocytes including peripheral blood leucocytes (PBL), thymus and spleen.
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