|Reactivity||Human, Mouse, Rat|
|Positive Control||Jurkat cell lysate|
|Application & Usage||Western Blot analysis (2-4 µg/ml). However, the optimal concentrations should be determined individually. Blocking peptide is available separately.|
|Formulation||200 µg (0.5 mg/ml) affinity purified rabbit anti-Lin28 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Lin28 antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
LIN28 is a highly conserved cytoplasmic RNAbinding protein. Lin28 has an N-terminal cold shock domain and two Cterminal (CCHC) zinc finger motif. It is expressed in embryonic stem cells and embryonic carcinoma cells. Lin28 is also found in adult cardiac and skeletal muscle.
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