|Application ||WB, IHC, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||71578 Da|
|Application & Usage||Western blot analysis (0.5-4 µg/ml) and in in immunoprecipitation (5-10 µg/ml). However, the optimal conditions should be determined individually. The affinity-purified rabbit antibody recognizes Wee1 protein from human, mouse and rat samples. Mouse small intestine tissue lysate can be used as a positive control|
|Other Names||WEE1hu, Wee1A kinase, DKFZp686I18166, FLJ16446, EC 18.104.22.168|
|Formulation||100 µg (0.5 mg/ml) affinity-purified rabbit anti-Wee1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Wee1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation (By similarity).|
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Provided below are standard protocols that you may find useful for product applications.
Wee1 is a nuclear tyrosine protein kinase. The protein plays a role in suppressing eukaryotes’ entry into mitosis by inactivating cdc2 kinase by inhibiting tyrosine phosphorylation. The predicted molecular weight of the kinase is 72 kDa. The kinase possesses tyrosine kinase activity, but structurally belongs to the serine/threonine protein kinase family.
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