|Reactivity||Human, Mouse, Rat|
|Calculated MW||22171 Da|
|Application & Usage||Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects a 20 kDa Bim/BOD in samples from human, mouse and rat origins. Jurkat cell lysate can be used as a positive control.|
|Other Names||Bcl-2-like protein 11, BCL2L11|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-Bim/BOD polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Bim/Bod Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Induces apoptosis and anoikis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than isoform BimEL, isoform BimL and isoform BimS. Isoform Bim-gamma induces apoptosis. Isoform Bim-alpha3 induces apoptosis possibly through a caspase-mediated pathway. Isoform BimAC and isoform BimABC lack the ability to induce apoptosis.|
|Cellular Location||Endomembrane system; Peripheral membrane protein. Note=Associated with intracytoplasmic membranes. Isoform BimL: Mitochondrion. Isoform Bim-alpha1: Mitochondrion.|
|Tissue Location||Isoform BimEL, isoform BimL and isoform BimS are the predominant isoforms and are widely expressed with tissue- specific variation. Isoform Bim-gamma is most abundantly expressed in small intestine and colon, and in lower levels in spleen, prostate, testis, heart, liver and kidney|
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Provided below are standard protocols that you may find useful for product applications.
Members of the Bcl-2 family proteins all contain Bcl-2 homology domains including BH1, BH2, BH3, and BH4. The BH3 domain is known to be important for the cell killing activity of Bcl-2 members including Bad, Bax, Bid, Bik, and the recently identified BH3 domain containing protein designated Bim/BOD. Bim/BOD interacts with diverse members of the Bcl-2 sub-family including Bcl-2, Bcl-x, and Bcl-w, to induce apoptosis. There are three splice variants: BimL, BimS, and BimEL, all of which share homology in their C-terminal BH3 domains. Of the isoforms, BimS is the most potent inducer of apoptosis. The mRNA of Bim is ubiquitously expressed in multiple tissues and cell lines.
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