|Application ||IHC, IF, FC, IP|
|Calculated MW||61377 Da|
|Application & Usage||Immunoprecipitation (2-8 µg/ml), Flow cytometry (2-8 µg/ml), Immunohistochemistry (2-8 µg/ml for both frozen and paraffin sections), Immunocytochemistry (2-8 µg/ml),. However, the optimal conditions should be determined individually. Recognizes the 70 kDa perforin in human and primate.|
|Other Names||Perforin-1, P1, Cytolysin, Lymphocyte pore-forming protein, PFP, PRF1, PFP|
|Formulation||100 µg (1 mg/ml) in PBS containing 1 mg/ml BSA and 1.5 mM thimerosal and 50% glycerol.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Perforin Antibody (Clone δG9) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.|
|Cellular Location||Cytoplasmic granule lumen. Secreted. Cell membrane; Multi-pass membrane protein. Endosome lumen. Note=Stored in cytoplasmic granules of cytolytic T-lymphocytes and secreted into the cleft between T-lymphocyte and target cell. Inserts into the cell membrane of target cells and forms pores. Membrane insertion and pore formation requires a major conformation change May be taken up via endocytosis involving clathrin-coated vesicles and accumulate in a first time in large early endosomes|
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Provided below are standard protocols that you may find useful for product applications.
Human perforin (cytolysin) is believed to be one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis. Perforin expression is constitutive on NK cells, but increases in resting XD8+ cytotoxic cells upon activation.
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