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Perforin Antibody (Clone δG9)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| IHC, IF, FC, IP |
|---|---|
| Primary Accession | P14222 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG2b |
| Clone Names | δG9 |
| Calculated MW | 61377 Da |
| Gene ID | 5551 |
|---|---|
| Application & Usage | Immunoprecipitation (2-8 µg/ml), Flow cytometry (2-8 µg/ml), Immunohistochemistry (2-8 µg/ml for both frozen and paraffin sections), Immunocytochemistry (2-8 µg/ml),. However, the optimal conditions should be determined individually. Recognizes the 70 kDa perforin in human and primate. |
| Other Names | Perforin-1, P1, Cytolysin, Lymphocyte pore-forming protein, PFP, PRF1, PFP |
| Target/Specificity | Perforin |
| Antibody Form | Liquid |
| Appearance | Colorless liquid |
| Formulation | 100 µg (1 mg/ml) in PBS containing 1 mg/ml BSA and 1.5 mM thimerosal and 50% glycerol. |
| Handling | The antibody solution should be gently mixed before use. |
| Reconstitution & Storage | -20 °C |
| Background Descriptions | |
| Precautions | Perforin Antibody (Clone δG9) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | PRF1 |
|---|---|
| Synonyms | PFP |
| Function | Pore-forming protein that plays a key role in granzyme- mediated programmed cell death, and in defense against virus-infected or neoplastic cells (PubMed:9058810, PubMed:9164947, PubMed:20889983, PubMed:24558045, PubMed:21037563). Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease (PubMed:9058810). Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores (PubMed:20889983, PubMed:21037563). Promotes cytolysis and apoptosis of target cells by mediating the passage and uptake of cytotoxic granzymes (PubMed:20038786, PubMed:20225066, PubMed:24558045, PubMed:32299851). Facilitates the delivery of cationic cargo protein, while anionic or neural proteins are not delivered efficiently (PubMed:24558045). Perforin pores allow the release of mature caspase-7 (CASP7) into the extracellular milieu (By similarity). |
| Cellular Location | Cytolytic granule. Secreted. Cell membrane; Multi-pass membrane protein. Endosome lumen. Note=Stored in cytolytic granules of cytolytic T-lymphocytes and secreted into the cleft between T- lymphocyte and target cell (PubMed:20038786). Inserts into the cell membrane of target cells and forms pores (PubMed:20889983). Membrane insertion and pore formation requires a major conformation change (PubMed:20889983). May be taken up via endocytosis involving clathrin- coated vesicles and accumulate in a first time in large early endosomes (PubMed:20038786). |
Citations (0)
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
Human perforin (cytolysin) is believed to be one of the major effector molecules used by cytotoxic T cells and NK cells to mediate targeted cell lysis. Perforin expression is constitutive on NK cells, but increases in resting XD8+ cytotoxic cells upon activation.
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