|Application ||WB, IHC, FC|
|Calculated MW||37822 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml) amd in Immunohistochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. The purified antibody detects both the proform (37-43 kDa) and the mature form (25 kDa) of cathepsin B. It reacts with human and mouse and in a lesser extent with rat samples. |
|Other Names||CTSB, CPSB, APPS, EC 18.104.22.168|
|Formulation||100 µg (0.2 mg/ml) affinity purified rabbit anti-cathepsin B polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Cathepsin B Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.|
|Cellular Location||Lysosome. Melanosome. Secreted, extracellular space. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV|
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Provided below are standard protocols that you may find useful for product applications.
Cathepsin B is a normal lysosomal cysteine proteinase that is expressed in all cells. It is synthesized as an inactive 43 kDa pro-enzyme which, by removal of a 62 amino acid propeptide, is activated to the single-chain form of 31 kDa or the two-chain form (25 and 5 kDa subunits). Matured cathepsin B is normally localized in the lysosomes where it functions in protein turnover. Cathepsin B is also shown capable of degrading extracellular matrix proteins. Elevated levels of cathepsin B correlate with malignancy s µggesting that this enzyme may be a useful prognostic marker for several types of human cancers.
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