|Application ||WB, IHC|
|Calculated MW||37564 Da|
|Positive Control||Western Blot: Jurkat cell lysate. IHC: Kidney tissue|
|Application & Usage||Western blotting (0.5-4 µg/ml), and Immunohistochemistry (5 µg/ml). However, the optimal conditions should be determined individually. Jurkat cell lysate can be used as a positive control. Detects both the proenzyme and the mature form of cathepsin L|
|Other Names||CTSL, CATL, CTSL1, MEP, EC 220.127.116.11|
|Formulation||100 µg (0.2 mg/ml) affinity purified rabbit anti-cathepsin L polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.1% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Cathepsin L Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Important for the overall degradation of proteins in lysosomes.|
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Provided below are standard protocols that you may find useful for product applications.
Cathepsin L is a normal lysosomal cysteine endoproteases that is expressed in all cells. The latent 39 kDa pro-Cathepsin L must be processed by cleavage of the 96 amino acid pro-peptide, to generate the active two-chain form made up of 21 and 5 kDa subunits. Mature Cathepsin L is normally localized in the lysosomes where it plays a major role in intracellular protein catabolism. The fact that Cathepsin L is also capable of degrading extracellular matrix proteins s µggests that it may play a role in invasion and metastasis and could be a useful prognostic indicator for cancer.
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