|Application ||WB, IHC|
|Calculated MW||37564 Da|
|Positive Control||Western Blot: Jurkat cell lysate. IHC: Kidney tissue|
|Application & Usage||Western blotting (0.5-4 µg/ml), and Immunohistochemistry (5 µg/ml). However, the optimal conditions should be determined individually. Jurkat cell lysate can be used as a positive control. Detects both the proenzyme and the mature form of cathepsin L|
|Other Names||CTSL, CATL, CTSL1, MEP, EC 188.8.131.52|
|Formulation||100 µg (0.2 mg/ml) affinity purified rabbit anti-cathepsin L polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.1% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Cathepsin L Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Important for the overall degradation of proteins in lysosomes.|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Cathepsin L is a normal lysosomal cysteine endoproteases that is expressed in all cells. The latent 39 kDa pro-Cathepsin L must be processed by cleavage of the 96 amino acid pro-peptide, to generate the active two-chain form made up of 21 and 5 kDa subunits. Mature Cathepsin L is normally localized in the lysosomes where it plays a major role in intracellular protein catabolism. The fact that Cathepsin L is also capable of degrading extracellular matrix proteins s µggests that it may play a role in invasion and metastasis and could be a useful prognostic indicator for cancer.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.