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ACAT1 Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | P17764 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 44695 Da |
| Gene ID | 25014 |
|---|---|
| Positive Control | Jurkat cell lysate |
| Application & Usage | The antibody can be used for Western blot analysis (1-4 µg/ml). However, the optimal conditions should be determined individually. Blocking peptide is available separately. |
| Other Names | Acetyl-CoA acetyltransferase mitochondrial |
| Target/Specificity | ACAT1 |
| Antibody Form | Liquid |
| Appearance | Colorless liquid |
| Formulation | 100 µg (0.5 mg/ml) affinity purified rabbit anti-ACAT1 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal. |
| Handling | The antibody solution should be gently mixed before use. |
| Reconstitution & Storage | -20 °C |
| Background Descriptions | |
| Precautions | ACAT1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Acat1 |
|---|---|
| Function | This is one of the enzymes that catalyzes the last step of the mitochondrial beta-oxidation pathway, an aerobic process breaking down fatty acids into acetyl-CoA. Using free coenzyme A/CoA, catalyzes the thiolytic cleavage of medium- to long-chain 3-oxoacyl-CoAs into acetyl-CoA and a fatty acyl-CoA shortened by two carbon atoms. The activity of the enzyme is reversible and it can also catalyze the condensation of two acetyl-CoA molecules into acetoacetyl-CoA. Thereby, it plays a major role in ketone body metabolism. |
| Cellular Location | Mitochondrion {ECO:0000250|UniProtKB:P24752}. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Acetyl-Coenzyme A acetyltransferase 1 (ACAT1) is a mitochondrial enzyme that catalyzes the formation of cholesterol esters from cholesterol and long chain fatty acyl-coenzyme A. ACAT1 may play an important role in the development of atherosclerosis due to cholesterol buildup in macrophage.
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