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Phospho-Bad Antibody

Rabbit Polyclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q61337.1
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Additional Information
Application & Usage Western blot analysis (1-2 µg/ml) and immunoprecipitation (5-20 µg/ml). However, the optimal conditions should be determined individually. The antibody detects 23 kDa Bad only when phosphorylated at Ser112.
Other Names BAD, phospho BBC2, phosphoBBC6, BAD (Phospho-Ser112), BCL2L8
Target/Specificity Bad
Antibody Form Liquid
Appearance Colorless liquid
Formulation 100 µg (0.2 mg/ml) peptide affinity purified rabbit anti-Phospho-Bad (Ser112) polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsPhospho-Bad Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Citations (0)

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Bad is a member of the Bcl-2 family protein that selectively dimerize with Bcl-xL and Bcl-2 resulting in cell death. Survival factors such as IL-3 can inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser 136. Phosphorylation at these sites results in the binding of Bad to 14-3-3 proteins and the inhibition of Bad binding to Bcl-xL and Bcl-2. Recently, Akt has also been shown to promote cell survival via its ability to phosphorylate Bad at Ser136.

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$ 325.00
Cat# ABV10212
(40 western blots)
Availability: 5-7days
Bulk Size
Seasonal Special on Bulk Order
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