|Application ||WB, IP|
|Calculated MW||39318 Da|
|Application & Usage||Western blot analysis (0.5-4 µg/ml) and immunoprecipitation. However, the optimal conditions should be determined individually.|
|Other Names||MAPKK3, MEK3, PRKMK3, MAP2K3, MKK3|
|Formulation||100 µg (0.2 mg/ml) protein A affinity purified rabbit anti-MKK3 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||MKK3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||MEK3, MKK3, PRKMK3, SKK2|
|Function||Dual specificity kinase. Is activated by cytokines and environmental stress in vivo. Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in the MAP kinase p38. Part of a signaling cascade that begins with the activation of the adrenergic receptor ADRA1B and leads to the activation of MAPK14.|
|Tissue Location||Abundant expression is seen in the skeletal muscle. It is also widely expressed in other tissues|
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Provided below are standard protocols that you may find useful for product applications.
MKK3 is a protein kinase that phosphorylates the p38 MAPK. Phosphorylation by MKK3 occurs on threonine and tyrosine residues and increases the activity of p38 to stimulate transcription factors ATF2 and Elk-1. MKK3, together with MKK6, serves as upstream regulators of p38 MAPK activation. A structural variant, MKK3b, has been identified that contains 29 more amino acids at its N-terminus.
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