RasGAP Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC, IP |
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Primary Accession | P20936 |
Reactivity | Human, Mouse, Rat, Bovine |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 116403 Da |
Gene ID | 5921 |
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Application & Usage | Western blotting (0.5-4 µg/ml) and in immunoprecipitation (10-20 µg/ml) and Immunohistochemistry (10-20 µg/ml). However, the optimal concentrations should be determined individually. Jurkat cell lysate can be used as a positive control. The antibody recognizes ~120 kDa Ras GAP of human, mouse, rat, and bovine origins. |
Other Names | RASGAP, p120GAP , RASA1, RASA, CMAVM, CM-AVM , PKWS , p120GAP, DKFZp434N071 |
Target/Specificity | RasGAP |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | 100 µg (0.2 mg/ml) protein A purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal. |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | RasGAP Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | RASA1 |
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Synonyms | GAP, RASA |
Function | Inhibitory regulator of the Ras-cyclic AMP pathway. Stimulates the GTPase of normal but not oncogenic Ras p21; this stimulation may be further increased in the presence of NCK1. |
Cellular Location | Cytoplasm. |
Tissue Location | In placental villi, detected only in the trophoblast layer (cytotrophoblast and syncytiotrophoblast). Not detected in stromal, endothelial or Hofbauer cells (at protein level) |
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Provided below are standard protocols that you may find useful for product applications.
Background
The mammalian proto-oncogene Ras encodes 21 kDa proteins (p21Ras) which exists in either a physiologically quiescent GDP-binding state or a GTPbinding signal-emitting state. Interaction of p21Ras with GTPase activating protein (GAP) can increase hydrolysis of p21Ras-bound GTP by as much as 1000-fold. The product of the neurofibromatosis type 1 gene (NF1) has also been shown to exhibit p21Ras GAP activity.
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