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DEDAF Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | Q8N488 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 24822 Da |
| Gene ID | 23429 |
|---|---|
| Application & Usage | Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. A549 or HepG2 cell lysate can be used as a positive control. A 32 kDa band should be detected. |
| Other Names | DEDAF , YEAF1 , AAP1 , APAP-1, RYBP, RING1 and YY1-binding protein; Death effector domain-associated factor; DED-associated factor; YY1 and E4TF1-associated factor 1; Apoptin-associating protein 1; APAP-1 |
| Target/Specificity | DEDAF |
| Antibody Form | Liquid |
| Appearance | Colorless liquid |
| Formulation | 100 µg (0.5 mg/ml) affinity purified rabbit anti-DEDAF polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal. |
| Handling | The antibody solution should be gently mixed before use. |
| Reconstitution & Storage | -20 °C |
| Background Descriptions | |
| Precautions | DEDAF Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | RYBP |
|---|---|
| Synonyms | DEDAF, YEAF1 |
| Function | Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1-like complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:25519132). Component of a PRC1-like complex that mediates monoubiquitination of histone H2A 'Lys-119' on the X chromosome and is required for normal silencing of one copy of the X chromosome in XX females. May stimulate ubiquitination of histone H2A 'Lys-119' by recruiting the complex to target sites (By similarity). Inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes (PubMed:19098711). May also regulate the ubiquitin-mediated proteasomal degradation of other proteins like FANK1 to regulate apoptosis (PubMed:14765135, PubMed:27060496). May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1 (PubMed:11953439). May bind to DNA (By similarity). May play a role in the repression of tumor growth and metastasis in breast cancer by down-regulating SRRM3 (PubMed:27748911). |
| Cellular Location | Nucleus. Cytoplasm. Nucleus, nucleoplasm {ECO:0000250|UniProtKB:Q8CCI5}. Note=Primarily found in the nucleus Detected in a punctate pattern likely to represent Polycomb group (PcG) bodies (By similarity). {ECO:0000250|UniProtKB:Q8CCI5} |
| Tissue Location | Down-regulated in breast cancer tissues and in several breast cancer cell lines (at protein level) (PubMed:27748911) Widely expressed with highest levels in lymphoid tissues and placenta |
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Provided below are standard protocols that you may find useful for product applications.
Background
Cell death signal are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. A novel protein that interacts with DED of caspase-8 and -10, and FADD was recently identified and designated DEDAF (for DED associated factor). DEDAF is identical to the transcriptional repressor RYBP. DEDAF interacts with FADD and a µgments the formation of CD95/FADD/caspase-8 complexes at the cytoplasm. DEDAF also interacts with DED-containing DNA biding protein (DEDD) in the nucleus, indicating it is involved in the regulation of both cytoplasmic and nuclear events of apoptosis.
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