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IkBalpha Antibody (Clone 6A055)
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IHC, IP |
|---|---|
| Primary Accession | P25963 |
| Reactivity | Human |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone Names | 6A055 |
| Calculated MW | 35609 Da |
| Gene ID | 4792 |
|---|---|
| Application & Usage | Western blotting (0.5-4 µg/ml) and immunoprecipitation (1-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects both phosphorylated and non-phosphorylated forms of IkBα. |
| Other Names | IkappaBalpha , MAD3, MAD-3, IKBA, NFKBI, IKB-alpha, I-kappa-B-alpha |
| Target/Specificity | IkBalpha |
| Antibody Form | Liquid |
| Appearance | Colorless liquid |
| Formulation | 100 µg (0.25 mg/ml) immunoaffinity purified mouse monoclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% thimerosal. |
| Handling | The antibody solution should be gently mixed before use. |
| Reconstitution & Storage | -20 °C |
| Background Descriptions | |
| Precautions | IkBalpha Antibody (Clone 6A055) is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | NFKBIA |
|---|---|
| Synonyms | IKBA, MAD3, NFKBI |
| Function | Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL (RELA/p65 and NFKB1/p50) dimers in the cytoplasm by masking their nuclear localization signals (PubMed:1493333, PubMed:7479976, PubMed:36651806). On cellular stimulation by immune and pro-inflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription (PubMed:7796813, PubMed:7628694, PubMed:7878466, PubMed:7479976). |
| Cellular Location | Cytoplasm. Nucleus. Note=Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export. |
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Provided below are standard protocols that you may find useful for product applications.
Background
NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/α, IKK2/β, and IKK3/g. External stimuli such TNF-α or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified. One of the first gene induced following NF-kB activation is IkBα.
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