|Application ||WB, IHC, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||35937 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml), immunoprecipitation, and Immunohistochemistry. However, the optimal conditions should be determined individually. The antibody detects annexin V from samples of human, mouse, and rat origin. Jurkat cell lysate, mouse small intestine and rat kidney tissue lysates can be used as positive controls.|
|Other Names||ANXA5, ANX5, ENX2, PP4|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-Annexin V polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Annexin V Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||ANX5, ENX2, PP4|
|Function||This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.|
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Provided below are standard protocols that you may find useful for product applications.
The annexin family of calcium-binding proteins is composed of at least ten mammalian genes. It is characterized by a conserved core domain which binds to phospholipids in a Calcium dependent manner and a unique amino terminal region which may confer binding specificity. Annexin family members have been implicated as regulators of such diverse processes as ion flux, endocytosis and exocytosis, and cellular adhesion. Annexin V is ubiquitously expressed at high levels in tissues and cells grown in culture.
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