|Reactivity||Human, Mouse, Rat|
|Calculated MW||44888 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody detects 62 kDa phosphorylated Elk-1 (Ser383) of human, mouse and rat origins.|
|Other Names||phosphoELK1, phospho ELK 1, phospho ELK- 1, elk-1, Oncogene Elk1, ELK1 member of ETS oncogene family|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Phospho-Elk-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcription factor that binds to purine-rich DNA sequences. Forms a ternary complex with SRF and the ETS and SRF motifs of the serum response element (SRE) on the promoter region of immediate early genes such as FOS and IER2. Induces target gene transcription upon JNK-signaling pathway stimulation (By similarity).|
|Tissue Location||Lung and testis.|
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Provided below are standard protocols that you may find useful for product applications.
Elk-1 is a transcription factor that binds the serum response element (SRE) and mediates gene activity in response to serum and growth factors. Elk-1 is phosphorylated by MAP kinase pathways and appears to be a direct target of activated MAP kinase. Biochemical studies indicate that Elk-1 is a good substrate for MAP kinase. The kinetics of Elk-1 phosphorylation and activation correlate with MAP kinase activity. Other studies have shown that Elk-1 (Ser383) is also a target of the stress-activated kinase SAPK/JNK.
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