ILK1 Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application
| WB, IHC |
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Primary Accession | O55222 |
Other Accession | NP_034692 |
Reactivity | Human, Mouse, Rat |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | Rabbit IgG |
Calculated MW | 51373 Da |
Gene ID | 16202 |
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Application & Usage | Western blotting (0.5-4 µg/ml), Immunohistochemistry (10-20 µg/ml, frozen & paraffin). However, the optimal concentrations should be determined individually. The antibody recognizes 59 kDa of ILK1 from samples of human, mouse, and rat origins. Reactivity to other species has not been determined. |
Other Names | p59ILK , ILK-1 , P59 , DKFZp686F1765 |
Target/Specificity | ILK1 |
Antibody Form | Liquid |
Appearance | Colorless liquid |
Formulation | 100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal. |
Handling | The antibody solution should be gently mixed before use. |
Reconstitution & Storage | -20 °C |
Background Descriptions | |
Precautions | ILK1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
Name | Ilk {ECO:0000312|MGI:MGI:1195267} |
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Function | Receptor-proximal protein kinase regulating integrin-mediated signal transduction. May act as a mediator of inside-out integrin signaling. Focal adhesion protein part of the complex ILK-PINCH. This complex is considered to be one of the convergence points of integrin- and growth factor-signaling pathway. Could be implicated in mediating cell architecture, adhesion to integrin substrates and anchorage-dependent growth in epithelial cells. Regulates cell motility by forming a complex with PARVB. Phosphorylates beta-1 and beta-3 integrin subunit on serine and threonine residues, but also AKT1 and GSK3B. |
Cellular Location | Cell junction, focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side {ECO:0000250|UniProtKB:Q13418}. Cytoplasm, myofibril, sarcomere {ECO:0000250|UniProtKB:Q13418}. Cell projection, lamellipodium |
Tissue Location | Highly expressed in lung, heart, kidney, liver, brain, spleen and skeletal muscle. Weakly expressed in testis |
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Provided below are standard protocols that you may find useful for product applications.
Background
ILKs (Integrin-linked kinases) in combination with integrins and growth factors regulate cell survival, cell cycle, cell-cell adhesion and cell motility. ILK functions as a scaffold bridging the extra-cellular matrix (ECM) and growth factor receptors to the actin cytoskeleton thro µgh interactions with integrin, PINCH (which links ILK to the RTKs via Nck2), CH-ILKBP and affixin. ILK phosphorylates several cellular targets including Akt, GSK-3, myosin light chain 2, as well as affixin. These phosphorylation events are key regulatory steps in modulating the activities of the targets.
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