|Application ||WB, IHC, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||89866 Da|
|Positive Control||Western Blot: 3T3 cell lysate and rat Kideny tissue lysate. IHC: Breast tissue|
|Application & Usage||Western blotting (2-10 µg/ml), immunoprecipitation (20 µg/ml) and Immunohistochemistry (5 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~95 kDa Stat5 of human, mouse and rat origins. Reactivity to other species has not been tested.|
|Other Names||Signal transducer and activator of transcription 5, stat -5, STAT5, STAT-5, STAT 5|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS).|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Stat5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and activates PRL-induced transcription.|
|Cellular Location||Cytoplasm. Nucleus. Note=Translocated into the nucleus in response to phosphorylation.|
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Provided below are standard protocols that you may find useful for product applications.
Membrane receptor signaling by various ligands induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Altho µgh early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 is activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.
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