|Application ||WB, IF|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||90831 Da|
|Application & Usage||Western blotting (1-4 µg/ml) and immunofluorescence (10-20 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~90 kDa phosphorylated Stat5 (Tyr694) of human, mouse, and rat origins. Reactivity to other species has not been tested. |
|Other Names||Anti-Stat-5, STAT-5|
|Formulation||100 µg (0.5 mg/ml) purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% sodium azide.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Phospho-Stat5 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.|
|Cellular Location||Cytoplasm. Nucleus. Note=Translocated into the nucleus in response to phosphorylation|
|Tissue Location||In the virgin, found in most tissues except brain and muscle. During lactation, abundantly found in mammary tissue, as well as in other secretory organs such as salivary gland and seminal vesicle|
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Provided below are standard protocols that you may find useful for product applications.
Membrane receptor signaling by various ligands induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Altho µgh early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 is activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.
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