|Reactivity||Human, Mouse, Rat|
|Calculated MW||52668 Da|
|Application & Usage||Western blotting (2-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes Presenilin 1 of human, mouse, and rat origins. Reactivity to other species has not been tested.|
|Other Names||PSEN1, Presenilin-1; PS-1; Protein S182; Presenilin-1 NTF subunit; Presenilin-1 CTF subunit; Presenilin-1 CTF12; PS1-CTF12|
|Formulation||100 µg (0.5 mg/ml) purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% sodium azide.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Presenilin 1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||AD3, PS1, PSNL1|
|Function||Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. Stimulates cell-cell adhesion though its association with the E-cadherin/catenin complex. Under conditions of apoptosis or calcium influx, cleaves E-cadherin promoting the disassembly of the E-cadherin/catenin complex and increasing the pool of cytoplasmic beta-catenin, thus negatively regulating Wnt signaling. May also play a role in hematopoiesis.|
|Cellular Location||Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cell surface. Note=Bound to NOTCH1 also at the cell surface. Colocalizes with CDH1/2 at sites of cell-cell contact Colocalizes with CTNNB1 in the endoplasmic reticulum and the proximity of the plasma membrane. Also present in azurophil granules of neutrophils|
|Tissue Location||Expressed in a wide range of tissues including various regions of the brain, liver, spleen and lymph nodes|
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Provided below are standard protocols that you may find useful for product applications.
Presenilin 1 and presenilin 2 are transmembrane proteins belonging to the presenilin family. Mutation of presenilin genes has been linked to early onset of Alzheimer’s disease, probably functioning thro µgh its associated g-secretase activity for amyloid-β protein processing. Endogenous presenilin mainly exists in a heterodimeric complex formed from the endoproteolytically processed N-terminal (34 kDa) and C-terminal (~20 kDa) fragments.
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