|Application ||WB, IHC|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||50981 Da|
|Application & Usage||Western blot analysis (1-2 µg/ml), and Immunohistochemistry (20-30 µg/ml). However, the optimal conditions should be determined individually. The phospho-GSK-3α/β (Ser21/9) antibody detects GSK-3 only when phosphorylated at Ser21 of GSK-3α or Ser9 of GSK-3β. |
|Other Names||DKFZp686D0638, GSK-3alpha|
|Formulation||100 µg (0.5 mg/ml) affinity-purified rabbit polyclonal phospho-GSK-3a(Ser21/9) polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% sodium azide.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Phospho-GSK-3a Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), CTNNB1/beta-catenin, APC and AXIN1. Requires primed phosphorylation of the majority of its substrates. Contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis. Regulates glycogen metabolism in liver, but not in muscle. May also mediate the development of insulin resistance by regulating activation of transcription factors. In Wnt signaling, regulates the level and transcriptional activity of nuclear CTNNB1/beta-catenin. Facilitates amyloid precursor protein (APP) processing and the generation of APP-derived amyloid plaques found in Alzheimer disease. May be involved in the regulation of replication in pancreatic beta-cells. Is necessary for the establishment of neuronal polarity and axon outgrowth. Through phosphorylation of the anti-apoptotic protein MCL1, may control cell apoptosis in response to growth factors deprivation.|
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Provided below are standard protocols that you may find useful for product applications.
GSK-3 is a serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 has been implicated in the regulation of cell fate and in the Wnt signaling pathway. GSK-3 plays an important role in the PI3 kinase and Akt mediated cell survival pathways, and its activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β.
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