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>   home   >   Products   >   Primary Antibodies   >   NHERF1/ EBP50 Antibody   

NHERF1/ EBP50 Antibody

Rabbit Polyclonal Antibody

Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession Q9JJ19
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 38830 Da
Additional Information
Gene ID 59114
Positive Control Rat kidney tissue lysate
Application & Usage Western Blot analysis (1-4 µg/ml).
Other Names Na(+)/H(+) exchange regulatory cofactor NHE-RF1, Ezrin-radixin-moesin-binding phosphoprotein 50, Regulatory cofactor of Na(+)/H(+) exchanger, Sodium-hydrogen exchanger regulatory factor 1, Solute carrier family 9 isoform A3 regulatory factor 1
Target/Specificity NHERF1/ EBP50
Antibody Form Liquid
Appearance Colorless liquid
Formulation 100 µg (0.5 mg/ml) affinity purified rabbit anti- NHERF1polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsNHERF1/ EBP50 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Slc9a3r1
Synonyms Nherf, Nherf1
Function Scaffold protein that connects plasma membrane proteins with members of the ezrin/moesin/radixin family and thereby helps to link them to the actin cytoskeleton and to regulate their surface expression. Necessary for recycling of internalized ADRB2. Was first known to play a role in the regulation of the activity and subcellular location of SLC9A3. Necessary for cAMP-mediated phosphorylation and inhibition of SLC9A3. Involved in sperm capacitation. May participate in the regulation of the chloride and bicarbonate homeostasis in spermatozoa. May enhance Wnt signaling. May participate in HTR4 targeting to microvilli (By similarity). Involved in the regulation of phosphate reabsorption in the renal proximal tubules (By similarity).
Cellular Location Cytoplasm. Apical cell membrane. Cell projection, filopodium Cell projection, ruffle. Cell projection, microvillus. Endomembrane system; Peripheral membrane protein. Note=Colocalizes with actin in microvilli-rich apical regions of the syncytiotrophoblast. Present in lipid rafts of T-cells Translocates from the cytoplasm to the apical cell membrane in a PODXL-dependent manner. Colocalizes with CFTR at the midpiece of sperm tail (By similarity).
Citations (0)

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NHE3 regulatory factor 1 (NHERF-1) is a multifunctional adaptor protein that interacts with NHE3 via its PDZ domains. The amino-terminal PDZ domain regulates the dimerization of NHERF-1 in vivo. G protein-coupled receptor kinase 6A phosphorylates NHERF-1 at Ser 289 via a PDZ domain-mediated interaction.

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$ 245.00
Cat# ABV10380
(40 western blots)
Availability: 5-7days
Bulk Size
Seasonal Special on Bulk Order
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