|Reactivity||Human, Mouse, Rat|
|Calculated MW||40926 Da|
|Positive Control||Rat kidney tissue lysate|
|Application & Usage||Western Blot analysis (1-4 µg/ml). However, the optimal concentrations should be determined individually. Blocking peptide is available separately.|
|Other Names||F-box only protein 32, Atrogin-1, Muscle atrophy F-box protein|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-Fbx32 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Fbx32/MAFbx/ATR-1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Substrate recognition component of a SCF (SKP1-CUL1-F- box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1 (By similarity).|
|Cellular Location||Cytoplasm. Nucleus. Note=Shuttles between Cytoplasm and the nucleus|
|Tissue Location||Specifically expressed in cardiac and skeletal muscle|
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Provided below are standard protocols that you may find useful for product applications.
Atrogin-1 (Atr1) is also known as Muscle Atrophy F-box (MAFbx). It is an E3 ubiquitin ligase that initiates ATPdependent ubiquitin-mediated proteolysis and promotes muscle atrophy. MAFbx is approximately a 43kDa protein.
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