|Reactivity||Human, Mouse, Rat, Bovine|
|Calculated MW||52225 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes ~52 kDa PPARα in human sample. A ~28 kDa cleavage fragment can also be detected in samples of human, mouse, and rat origins.|
|Other Names||PPARA , PPAR , OTTHUMP00000042872 , hPPAR , MGC2452 , OTTHUMP00000028713 , NR1C1 , PPAR-alpha , MGC2237|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||PPAR-alpha Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Ligand-activated transcription factor. Key regulator of lipid metabolism. Activated by the endogenous ligand 1-palmitoyl- 2-oleoyl-sn-glycerol-3-phosphocholine (16:0/18:1-GPC). Activated by oleylethanolamide, a naturally occurring lipid that regulates satiety. Receptor for peroxisome proliferators such as hypolipidemic drugs and fatty acids. Regulates the peroxisomal beta-oxidation pathway of fatty acids. Functions as transcription activator for the ACOX1 and P450 genes. Transactivation activity requires heterodimerization with RXRA and is antagonized by NR2C2. May be required for the propagation of clock information to metabolic pathways regulated by PER2.|
|Tissue Location||Skeletal muscle, liver, heart and kidney.|
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Provided below are standard protocols that you may find useful for product applications.
PPAR (Peroxisome proliferator-activated receptor) is a member of the nuclear hormone receptor superfamily and functions as a transcriptional activator. PPARα is preferentially expressed in liver, skeletal muscle, heart and kidney. PPARα is involved in regulation of lipid homeostasis. Its ligands include fatty acids, NSAIDs, prostaglandins, leukotriene B4, etc. PPARα transcriptionally regulates a variety of genes for enzymes and proteins involved in fatty acid metabolism and oxidation.
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