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HDAC6 Antibody

Rabbit Polyclonal Antibody

     
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Product Information
Application
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • E=ELISA
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
WB, IHC
Primary Accession Q9Z2V5
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 125787 Da
Additional Information
Gene ID 15185
Application & Usage Western blotting (0.5-4 µg/ml) and Immunohistochemistry (20 µg/ml). However, the optimal conditions should be determined individually. The antibody detects ~134 kDa HDAC-6. Jurkat cell lysate can be used as a positive control. The antibody does not cross-react with other HDAC proteins including HDAC1, 2, 3, 4, 5, 7, and 8.
Other Names HD6, HDAC-6, histone deacetylase 6, JM21
Target/Specificity HDAC6
Antibody Form Liquid
Appearance Colorless liquid
Formulation 100 µg (0.2 mg/ml) affinity purified rabbit anti-HDAC6 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsHDAC6 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Hdac6 {ECO:0000312|MGI:MGI:1333752}
Function Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4) (PubMed:9891014). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events (PubMed:9891014). Histone deacetylases act via the formation of large multiprotein complexes (PubMed:9891014). In addition to histones, deacetylates other proteins, such as CTTN, tubulin and SQSTM1 (PubMed:19893491, PubMed:27737934). Plays a central role in microtubule-dependent cell motility by mediating deacetylation of tubulin (PubMed:19893491, PubMed:27737934). Required for cilia disassembly; via deacetylation of alpha-tubulin (By similarity). Promotes deacetylation of CTTN, leading to actin polymerization, promotion of autophagosome-lysosome fusion and completion of autophagy (By similarity). Promotes odontoblast differentiation following IPO7-mediated nuclear import and subsequent repression of RUNX2 expression (PubMed:35922041). In addition to its protein deacetylase activity, plays a key role in the degradation of misfolded proteins: when misfolded proteins are too abundant to be degraded by the chaperone refolding system and the ubiquitin- proteasome, mediates the transport of misfolded proteins to a cytoplasmic juxtanuclear structure called aggresome (By similarity). Probably acts as an adapter that recognizes polyubiquitinated misfolded proteins and target them to the aggresome, facilitating their clearance by autophagy (PubMed:22819792).
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton. Nucleus. Perikaryon Cell projection, dendrite. Cell projection, axon. Cell projection, cilium {ECO:0000250|UniProtKB:Q9UBN7}. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000250|UniProtKB:Q9UBN7} Cytoplasm, cytoskeleton, cilium basal body {ECO:0000250|UniProtKB:Q9UBN7}. Note=It is mainly cytoplasmic, where it is associated with microtubules.
Tissue Location Expressed in neurons of the cortex. Expressed in Purkinje cells. Detected in keratinocytes (at protein level)
Research Areas
Citations (0)
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Background

Human HDAC6 (1215 a.a. residues) possesses two separate putative catalytic domains, both of which are fully functional and contribute independently to the overall activity of HDAC6. A very potent NES is present at the amino-terminus of HDAC6, which was found to play an important role in regulating the shuttling of HDAC6 protein between cytoplasm and nucleus. The shuttling process may be a critical regulatory mechanism of HDAC6 function. HDAC6 may participate in coordinating expression of a group of genes involved in the remoldeling of chromatin during cell differentiation.

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Discontinued
Cat# ABV10451-30
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