|Application ||WB, IHC, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||66655 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml) , Immunohistochemistry (paraffin, 10-20 µg/ml) and immunoprecipitation (10-20 µg/ml). However, the optimal conditions should be determined individually.|
|Other Names||SELE , LECAM2 , ESEL , ELAM , ELAM-1 , CD62E , ELAM1|
|Formulation||100 µg (0.5mg/ml) protein A purified rabbit anti-E-Selectin polyclonal antibody in phosphate-buffered saline (PBS) containing 0.02% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||E-Selectin Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.|
|Cellular Location||Cell membrane; Single-pass type I membrane protein|
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Provided below are standard protocols that you may find useful for product applications.
Selectins are a family of carbohydrate-binding proteins involved in mediating cellular interactions with leukocytes. E-Selectin (also called ELMA-1, CD62E) expressed on endothelial cells. L-Selectin (also called LECAM-1) is expressed on the majority of B and naïve T cells and on most monocytes, neurophils and eosinophils. P-Selectin (also called GMP-140) expressed on activated platelets and endothelial cells. Selectins interact with specific carbohydrates expressed by activated endothelial cells, monocytes, and neutrophils.
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