|Application ||WB, IHC, IP|
|Reactivity||Human, Mouse, Rat|
|Calculated MW||21642 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml), Immunoprecipitation (5-10 µg/ml) and and Immunohistochemistry (5 µg/ml) (Frozen and paraffin sections). However, the optimal conditions should be determined individually. Other applications have not been determined.|
|Other Names||SDC4 , SYND4|
|Formulation||100 µg (0.5 mg/ml) Protein A purified rabbit anti-Syndecan-4 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Syndecan-4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Cell surface proteoglycan that bears heparan sulfate. Regulates exosome biogenesis in concert with SDCBP and PDCD6IP (PubMed:22660413).|
|Cellular Location||Isoform 1: Membrane; Single- pass type I membrane protein. Secreted. Note=Shedding of the ectodomain produces a soluble form.|
|Tissue Location||Expressed in epithelial and fibroblastic cells.|
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Provided below are standard protocols that you may find useful for product applications.
Syndecans are type I integral membrane proteoglycans that are involved in cell-extracellular matrix adhesion and growth factor binding. Syndecan-1 is an matrix receptor which binds to Collagens, Fibronectin and Thrombospondin. Syndecan-1 and Syndecan-3 interact with MK (midkine), a growth/differentiation factor involved in embryogenesis. Syndecan-2 is highly expressed at areas of high morphogenetic activity, such as epithelial-mesenchymal interfaces and the prechondrogenic and preosteogenic mesenchymal condensations. Syndecan-4 functions cooperatively with integrins in the processes of cell spreading and focal adhesion assembly.
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