|Application ||WB, IHC, E, IP|
|Calculated MW||515605 Da|
|Application & Usage||Western blotting (0.5-1 µg/ml), immunoprecipitation (5-10 µg/ml), Immunohistochemistry (5-10 µg/ml) and Elisa (0.5-1 µg/ml). However, the optimal conditions should be determined individually. The antibody reacts with human apoLipoprotein B. It shows negligible cross-reactivity with type A-I, A-II, C-I, C-II, C-III, E, and J apoLipoproteins (<1%).|
|Other Names||Apolipoprotein B-100, Apo B-100, Apolipoprotein B-48, Apo B-48, APOB|
|Formulation||100 µg (1 mg/ml) antigen affinity purified goat anti-apoLipoprotein B polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 0.01% thymersol.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||ApoLipoprotein B Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor.|
|Cellular Location||Cytoplasm. Secreted|
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Provided below are standard protocols that you may find useful for product applications.
Apolipoprotein B consists of a single polypeptide chain with a molecular weight of 549 kDa and is mostly synthesised in the liver. It is a major apolipoprotein of very low density, intermediate density and low density lipoproteins (LDL) as well as being a major component of lipoprotein (a). Apolipoprotein B is a ligand for the LDL receptor and elevated levels are associated with premature atherosclerosis. Normal plasma apolipoprotein B levels are around 800 mg/L.
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