Foundational characteristics of cancer include proliferation, angiogenesis, migration, evasion of apoptosis, and cellular immortality. Find key markers for these cellular processes and antibodies to detect them.
The SUMOplot™ Analysis Program predicts and scores sumoylation sites in your protein. SUMOylation is a post-translational modification involved in various cellular processes, such as nuclear-cytosolic transport, transcriptional regulation, apoptosis, protein stability, response to stress, and progression through the cell cycle.
The Autophagy Receptor Motif Plotter predicts and scores autophagy receptor binding sites in your protein. Identifying proteins connected to this pathway is critical to understanding the role of autophagy in physiological as well as pathological processes such as development, differentiation, neurodegenerative diseases, stress, infection, and cancer.
Bin1 Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | O08539 |
| Other Accession | BC065160 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 64470 Da |
| Gene ID | 30948 |
|---|---|
| Application & Usage | Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. Other applications have not been tested. The antibody recognizes ~65 kDa band in samples from human, mouse and rat origins. A cleavage fragment (~45 kDa) can also be detected in apoptotic samples. Reactivity to other species has not been tested. |
| Other Names | BIN1 , AMPH2 , amphiphysin-like , MGC10367 , DKFZp547F068 , AMPHL , SH3P9 |
| Target/Specificity | Bin1 |
| Antibody Form | Liquid |
| Appearance | Colorless liquid |
| Formulation | 100 µg (0.2mg/ml) immunoaffinity purified rabbit anti-Bin-1 polyclonal antibody in phosphate-buffered saline (PBS) containing 0.1% BSA, 30% glycerol, and 0.01% thimerosal. |
| Handling | The antibody solution should be gently mixed before use. |
| Reconstitution & Storage | -20 °C |
| Background Descriptions | |
| Precautions | Bin1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Bin1 |
|---|---|
| Synonyms | Amphl, Sh3p9 |
| Function | Is a key player in the control of plasma membrane curvature, and membrane shaping and remodeling. Required in muscle cells for the formation of T-tubules, tubular invaginations of the plasma membrane that function in depolarization-contraction coupling. Required in muscle cells for the formation of T-tubules, tubular invaginations of the plasma membrane that function in depolarization-contraction coupling (PubMed:12183633). Is a negative regulator of endocytosis (By similarity). Is also involved in the regulation of intracellular vesicles sorting, modulation of BACE1 trafficking and the control of amyloid-beta production (PubMed:12668730, PubMed:27179792). In neuronal circuits, endocytosis regulation may influence the internalization of PHF-tau aggregates (By similarity). May be involved in the regulation of MYC activity and the control cell proliferation (By similarity). |
| Cellular Location | Nucleus {ECO:0000250|UniProtKB:O00499}. Cytoplasm. Endosome Cell membrane, sarcolemma, T-tubule {ECO:0000250|UniProtKB:O08839} |
| Tissue Location | Isoform 1 is expressed mainly in the brain. Isoform 2 is widely expressed. |
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abcepta to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
info@abcepta.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
Background
BIN-1 is a tumor suppressor protein that was identified in a genetic screen for proteins that interact with the MCY oncoprotein. BIN-1 inhibits the oncogenic activity of MYC, possibly thro µgh activating or facilitating apoptosis. BIN is a short-lived protein with a half-life of about 2 hours.
If you have used an Abcepta product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at tech@abcepta.com.
Ordering Information
Other Products
Shipping Information
