|Calculated MW||52712 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. Mouse small intestine and rat kidney tissue lysates can be used as positive controls.|
|Other Names||HNF4A , TCF , MODY 1 , MODY , TCF14 , FLJ39654 , HNF4 , HNF 4|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||HNF-4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||Hnf-4, Hnf4, Nr2a1, Tcf14|
|Function||Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1- antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1- alpha. May be essential for development of the liver, kidney and intestine.|
|Tissue Location||Liver, kidney and intestine.|
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HNF-4 (Hepatocyte nuclear factor 4), a member of the steroid/thyroid nuclear receptor superfamily, is a transcriptional factor which expresses in the liver, intestine, kidney, and pancreatic cells. It contains several functional domains: a ligand-independent activation domain (AF1), a zinc finger DNA binding domain, and a ligand-dependent activation domain (AF2). HNF-4 binds to a specific DNA element as a homodimer and regulates the expression of many genes, involved in glucose, fatty acid, and cholesterol metabolisms.
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