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HNF-4 Antibody

Rabbit Polyclonal Antibody

  • WB - HNF-4 Antibody ABV10521
    Western blot analysis of HNF-4 with mouse small intestine tissue lysate.
Product Information
  • Applications Legend:
  • WB=Western Blot
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin-embedded Sections)
  • IHC-F=Immunohistochemistry (Frozen Sections)
  • IF=Immunofluorescence
  • FC=Flow Cytopmetry
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • IP=Immunoprecipitation
  • DB=Dot Blot
  • CHIP=Chromatin Immunoprecipitation
  • FA=Fluorescence Assay
  • IEM=Immunoelectronmicroscopy
  • EIA=Enzyme Immunoassay
Primary Accession P22449
Other Accession EDL96573
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit IgG
Calculated MW 52712 Da
Additional Information
Gene ID 25735
Application & Usage Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. Mouse small intestine and rat kidney tissue lysates can be used as positive controls.
Other Names HNF4A , TCF , MODY 1 , MODY , TCF14 , FLJ39654 , HNF4 , HNF 4
Target/Specificity HNF 4
Antibody Form Liquid
Appearance Colorless liquid
Formulation 100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
Handling The antibody solution should be gently mixed before use.
Reconstitution & Storage -20 °C
Background Descriptions
PrecautionsHNF-4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Hnf4a
Synonyms Hnf-4, Hnf4, Nr2a1, Tcf14
Function Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1- antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1- alpha. May be essential for development of the liver, kidney and intestine.
Cellular Location Nucleus.
Tissue Location Liver, kidney and intestine.
Citations (0)

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HNF-4 (Hepatocyte nuclear factor 4), a member of the steroid/thyroid nuclear receptor superfamily, is a transcriptional factor which expresses in the liver, intestine, kidney, and pancreatic cells. It contains several functional domains: a ligand-independent activation domain (AF1), a zinc finger DNA binding domain, and a ligand-dependent activation domain (AF2). HNF-4 binds to a specific DNA element as a homodimer and regulates the expression of many genes, involved in glucose, fatty acid, and cholesterol metabolisms.

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$ 245.00
Cat# ABV10521
(40 western blots)
Availability: 5-7days
Bulk Size
Seasonal Special on Bulk Order
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