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Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | P22449 |
| Other Accession | EDL96573 |
| Reactivity | Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 52712 Da |
| Gene ID | 25735 |
|---|---|
| Application & Usage | Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. Mouse small intestine and rat kidney tissue lysates can be used as positive controls. |
| Other Names | HNF4A , TCF , MODY 1 , MODY , TCF14 , FLJ39654 , HNF4 , HNF 4 |
| Target/Specificity | HNF 4 |
| Antibody Form | Liquid |
| Appearance | Colorless liquid |
| Formulation | 100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal. |
| Handling | The antibody solution should be gently mixed before use. |
| Reconstitution & Storage | -20 °C |
| Background Descriptions | |
| Precautions | HNF-4 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | Hnf4a |
|---|---|
| Synonyms | Hnf-4, Hnf4, Nr2a1, Tcf14 |
| Function | Transcriptionally controlled transcription factor. Binds to DNA sites required for the transcription of alpha 1- antitrypsin, apolipoprotein CIII, transthyretin genes and HNF1- alpha. May be essential for development of the liver, kidney and intestine. |
| Cellular Location | Nucleus. |
| Tissue Location | Liver, kidney and intestine. |
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Provided below are standard protocols that you may find useful for product applications.
Background
HNF-4 (Hepatocyte nuclear factor 4), a member of the steroid/thyroid nuclear receptor superfamily, is a transcriptional factor which expresses in the liver, intestine, kidney, and pancreatic cells. It contains several functional domains: a ligand-independent activation domain (AF1), a zinc finger DNA binding domain, and a ligand-dependent activation domain (AF2). HNF-4 binds to a specific DNA element as a homodimer and regulates the expression of many genes, involved in glucose, fatty acid, and cholesterol metabolisms.
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