|Reactivity||Human, Mouse, Rat|
|Calculated MW||18016 Da|
|Application & Usage||Western blotting (2-6 µg/ml). However, the optimal conditions should be determined individually. Jurkat cell lysate can be used as positive controls. The antibody detects a ~17 kDa BIK in samples from human, mouse, and rat origins. A ~15 kDa cleavage fragment can also be detected|
|Other Names||BCL2 interacting killer, Apoptosis inducer NBK, BIP 1, BIP, bik|
|Formulation||100 µg (0.5 mg/ml) Protein A affinity purified rabbit anti-BIK polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||BIK Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Accelerates programmed cell death. Association to the apoptosis repressors Bcl-X(L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact with BAX.|
|Cellular Location||Endomembrane system; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein. Note=Around the nuclear envelope, and in cytoplasmic membranes|
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Provided below are standard protocols that you may find useful for product applications.
BIK is a member of Pro-apoptotic Bcl-2 family proteins. Altho µgh Bik lacks Bcl-2 homologus region, BH1 and BH2, BIK interacts with Bcl-2 and Bcl-xL. BIK has been shown to induce apoptosis by activating caspase-9.
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