|Reactivity||Human, Mouse, Rat|
|Calculated MW||18016 Da|
|Application & Usage||Western blotting (2-6 µg/ml). However, the optimal conditions should be determined individually. Jurkat cell lysate can be used as positive controls. The antibody detects a ~17 kDa BIK in samples from human, mouse, and rat origins. A ~15 kDa cleavage fragment can also be detected|
|Other Names||BCL2 interacting killer, Apoptosis inducer NBK, BIP 1, BIP, bik|
|Formulation||100 µg (0.5 mg/ml) Protein A affinity purified rabbit anti-BIK polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||BIK Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Accelerates programmed cell death. Association to the apoptosis repressors Bcl-X(L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact with BAX.|
|Cellular Location||Endomembrane system; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein. Note=Around the nuclear envelope, and in cytoplasmic membranes|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
BIK is a member of Pro-apoptotic Bcl-2 family proteins. Altho µgh Bik lacks Bcl-2 homologus region, BH1 and BH2, BIK interacts with Bcl-2 and Bcl-xL. BIK has been shown to induce apoptosis by activating caspase-9.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.