|Reactivity||Human, Mouse, Rat|
|Calculated MW||48933 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects 47 kDa TUCAN in samples from human, mouse, and rat origins. Jurkat cell lysate, mouse small intestine and rat kidney tissue lysates can be used as positive controls.|
|Other Names||DACAR , NDPP1 , TUCAN , CARDINAL , KIAA0955 , MGC57162 , DKFZp779L0366|
|Formulation||100 µg (0.2 mg/ml) Protein A affinity purified rabbit anti-TUCAN polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5 BSA and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||TUCAN/CARD8 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Inhibits NF-kappa-B activation. May participate in a regulatory mechanism that coordinates cellular responses controlled by NF-kappa-B transcription factor. May be a component of the inflammasome, a protein complex which also includes PYCARD, NALP2 and CASP1 and whose function would be the activation of proinflammatory caspases.|
|Cellular Location||Cytoplasm. Nucleus|
|Tissue Location||High expression in lung, ovary, testis and placenta. Lower expression in heart, kidney and liver. Also expressed in spleen, lymph node and bone marrow|
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TUCAN (Tumor Up-regulated Card-containing Antagonist of Caspase-Nine) is a CARD (Caspase-Associated Recruitment Domains) containing protein that binds and suppresses activation of pro-Caspase-9 and found to be overexpressed in some types of cancers. TUCAN binds specifically to pro-caspase-9 and interferes with its interaction with Apaf-1, thereby suppressing apoptosis signaling in the mitochondrial/cytochrome c pathway.
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