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TUCAN/CARD8 Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB |
|---|---|
| Primary Accession | Q9Y2G2 |
| Reactivity | Human, Mouse, Rat |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 60652 Da |
| Gene ID | 22900 |
|---|---|
| Application & Usage | Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody detects 47 kDa TUCAN in samples from human, mouse, and rat origins. Jurkat cell lysate, mouse small intestine and rat kidney tissue lysates can be used as positive controls. |
| Other Names | DACAR , NDPP1 , TUCAN , CARDINAL , KIAA0955 , MGC57162 , DKFZp779L0366 |
| Target/Specificity | TUCAN |
| Antibody Form | Liquid |
| Appearance | Colorless liquid |
| Formulation | 100 µg (0.2 mg/ml) Protein A affinity purified rabbit anti-TUCAN polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5 BSA and 0.01% thimerosal. |
| Handling | The antibody solution should be gently mixed before use. |
| Reconstitution & Storage | -20 °C |
| Background Descriptions | |
| Precautions | TUCAN/CARD8 Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
| Name | CARD8 {ECO:0000303|PubMed:11821383, ECO:0000312|HGNC:HGNC:17057} |
|---|---|
| Function | Inflammasome sensor, which mediates inflammasome activation in response to various pathogen-associated signals, leading to subsequent pyroptosis of CD4(+) T-cells and macrophages (PubMed:11821383, PubMed:11408476, PubMed:15030775, PubMed:32840892, PubMed:32051255, PubMed:33542150, PubMed:34019797, PubMed:36357533). Inflammasomes are supramolecular complexes that assemble in the cytosol in response to pathogens and other damage-associated signals and play critical roles in innate immunity and inflammation (PubMed:11821383, PubMed:11408476, PubMed:15030775, PubMed:36357533). Acts as a recognition receptor (PRR): recognizes specific pathogens and other damage-associated signals, such as HIV-1 protease activity or Val- boroPro inhibitor, and mediates CARD8 inflammasome activation (PubMed:32840892, PubMed:33542150, PubMed:36357533). In response to pathogen-associated signals, the N-terminal part of CARD8 is degraded by the proteasome, releasing the cleaved C-terminal part of the protein (Caspase recruitment domain-containing protein 8, C-terminus), which polymerizes to initiate the formation of the inflammasome complex: the CARD8 inflammasome directly recruits pro-caspase-1 (proCASP1) independently of PYCARD/ASC and promotes caspase-1 (CASP1) activation, which subsequently cleaves and activates inflammatory cytokines IL1B and IL18 and gasdermin-D (GSDMD), leading to pyroptosis (PubMed:33053349, PubMed:32840892, PubMed:32051255, PubMed:33542150, PubMed:36357533). Ability to sense HIV-1 protease activity leads to the clearance of latent HIV-1 in patient CD4(+) T-cells after viral reactivation; in contrast, HIV-1 can evade CARD8-sensing when its protease remains inactive in infected cells prior to viral budding (PubMed:33542150). Also acts as a negative regulator of the NLRP3 inflammasome (PubMed:24517500). May also act as an inhibitor of NF- kappa-B activation (PubMed:11551959, PubMed:12067710). |
| Cellular Location | Cytoplasm. Nucleus |
| Tissue Location | High expression in lung, ovary, testis and placenta (PubMed:11551959). Lower expression in heart, kidney and liver (PubMed:11551959). Also expressed in spleen, lymph node and bone marrow (PubMed:11821383). |
Research Areas
Citations (0)
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Application Protocols
Provided below are standard protocols that you may find useful for product applications.
Background
TUCAN (Tumor Up-regulated Card-containing Antagonist of Caspase-Nine) is a CARD (Caspase-Associated Recruitment Domains) containing protein that binds and suppresses activation of pro-Caspase-9 and found to be overexpressed in some types of cancers. TUCAN binds specifically to pro-caspase-9 and interferes with its interaction with Apaf-1, thereby suppressing apoptosis signaling in the mitochondrial/cytochrome c pathway.
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