|Reactivity||Human, Mouse, Rat|
|Calculated MW||179145 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. Jurkat cell lysate can also be detected. Reactivity to other species has not been tested.|
|Other Names||LRP-5 , LRP -6|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||LRP-5/6 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor- ligand complexes into ribosome-sized signalsomes. Cell-surface coreceptor of Wnt/beta-catenin signaling, which plays a pivotal role in bone formation. The Wnt-induced Fzd/LRP6 coreceptor complex recruits DVL1 polymers to the plasma membrane which, in turn, recruits the AXIN1/GSK3B-complex to the cell surface promoting the formation of signalsomes and inhibiting AXIN1/GSK3- mediated phosphorylation and destruction of beta-catenin. Appears be required for postnatal control of vascular regression in the eye. Required for posterior patterning of the epiblast during gastrulation.|
|Cellular Location||Membrane; Single-pass type I membrane protein. Endoplasmic reticulum. Note=Chaperoned to the plasma membrane by MESD.|
|Tissue Location||Widely expressed, with the highest level of expression in the liver and in aorta|
Thousands of laboratories across the world have published research that depended on the performance of antibodies from Abgent to advance their research. Check out links to articles that cite our products in major peer-reviewed journals, organized by research category.
email@example.com, and receive a free "I Love Antibodies" mug.
Provided below are standard protocols that you may find useful for product applications.
LDL-related proteins LRP-5 and LRP-6. are members of the LDL receptor superfamily, which were found to be involved in the canonical Wnt signaling pathway. Mammalian LRP-6 was shown to bind to Wnt-1 and enhance Induced-induced developmental processes in Xenopus embryos. Mice lacking LRP-6 exhibited developmental defects that are similar to those caused by deficiencies in various Want proteins. Recent work also shows that Want proteins induce the binding of LRP-5 to Axin, leading Axin degradation and β-catenin stabilization.
If you have used an Abgent product and would like to share how it has performed, please click on the "Submit Review" button and provide the requested information. Our staff will examine and post your review and contact you if needed.
If you have any additional inquiries please email technical services at firstname.lastname@example.org.