|Application ||WB, IHC|
|Calculated MW||33394 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml) and Immunohistochemistry (5 µg/ml). However, the optimal conditions should be determined individually. The antibody detects a 33 kDa human Tim-3 on SDS-PAGE immunoblots. Other applications have not been determined.|
|Other Names||tim-3, tim3, tim 3, TIMD3, TIMD-3, T-cell immunoglobulin and mucin domain-containing protein 3, T-cell membrane protein 3|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||Tim-3 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Regulates macrophage activation. Inhibits T-helper type 1 lymphocyte (Th1)-mediated auto- and alloimmune responses and promotes immunological tolerance. May be also involved in T-cell homing. Receptor for LGALS9.|
|Cellular Location||Membrane; Single-pass type I membrane protein|
|Tissue Location||T-helper type 1 lymphocyte (Th1)-specific.|
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As a member of the T cell immunoglobulin and mucin domain (TIM) family, Tim-3 is reported to promote or terminate Th1 immunity and is thought to influence a range of inflammatory conditions. It is constitutively expressed in a subset of murine IFNgamma-secreting Th1 cells, but not Th2 cells, in both human and mice. Additionally, Tim-3 is expressed dendritic cells, and by a subset of CD4+ cells where its levels of expression increase upon anti-CD3/28 stimulation. This molecule is also highly expressed in in vitro polarized Th1 cells, and at lower levels in Th17 cells. It is known to synergize with Toll-like receptors and is thought to regulate Th1 and Th17 cytokine secretion.
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