|Calculated MW||22244 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually.|
|Other Names||O-6-methylguanine-DNA methyltransferase, O-6-methylguanine-DNA-alkyltransferase, Methylguanine DNA methyltransferase , Methylated DNA protein cysteine methyltransferase|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-MGMT polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||MGMT Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.|
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Provided below are standard protocols that you may find useful for product applications.
MGMT (O-6-methylguanine-DNA methyltransferase) is a DNA repair protein that response to DNA damage by removing methly or alkyl groups from the O6 position of guanine. MGMT protects normal cells from cytotoxic effects and is important in dr µg resistance to alkylating agents.
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