|Calculated MW||23826 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Other applications have not been tested. The antibody detects ~30 kDa SOCS1 on SDS-PAGE immunoblots. Blocking peptide is available separately.|
|Other Names||CIS1 , CISH1 , JAB , JAK binding protein , SOCS-1 , SOCS1 , SSI-1 , SSI1 , TIP3|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||SOCS1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein- tyrosine activity (By similarity). Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival. Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2 (By similarity). SOCS1 appears to be a negative regulator in IGF1R signaling pathway (By similarity).|
|Cellular Location||Nucleus. Cytoplasmic vesicle. Note=Detected in perinuclear cytoplasmic vesicles upon interaction with FGFR3.|
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Provided below are standard protocols that you may find useful for product applications.
The Suppressor of cytokine signaling (SOCS) and cytokine-inducible SH2 proteins are a family of intracellular proteins that regulate responses to cytokines. SOCS1 suppresses dendritic cell and T cell hyperactivation in response to cytokine signaling by inhibiting the JAK tyrosine kinase. JAK tyrosine kinase activity is important for type I and II cytokine receptors to initiate signaling, and its activity is inhibited by direct binding of SOCS1 to the catalytic domain of the kinase. SOCS1 also has E3 ubiquitin protein ligase activity that results in the polyubiquitination of its target proteins and subsequent degradation by the proteosome.
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