|Reactivity||Human, Mouse, Rat|
|Calculated MW||18414 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Other applications have not been determined.|
|Other Names||APG10 , APG10L, ATG10 , DKFZP586I0418 , FLJ13954 , pp12616|
|Formulation||0.5 mg/ml affinity purified rabbit anti-APG10 in PBS containing 30% glycerol, 0.5 mg/ml BSA and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||APG10/ATG10 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
Autophagy, the process of bulk degradation of cellular proteins thro µgh an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) thro µgh phosphorylation of autophagy protein APG1. Another member of the autophagy protein family is APG10 (Also called ATG10, APG10L), an E2-like enzyme involved in two ubiquitin-like modifications essential for autophagosome formation: ATG12-ATG5 conj µgation and the modification of a soluble form of MAP-LC3, a homolog of yeast Apg8, to a membrane-bound form. ATG10 has also been shown to interact with ATG12 in human embryonic kidney cells in the presence of ATG7. Multiple isoforms of ATG10 are known to exist.
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