|Reactivity||Human, Mouse, Rat|
|Calculated MW||15207 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually.|
|Other Names||APG12 , APG12L , ATG12 , HAPG12|
|Formulation||0.5 mg/ml affinity purified rabbit anti-APG12 in PBS containing 30% glycerol, 0.5 mg/ml BSA and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||APG12/ATG12 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. The ATG12-ATG5 conjugate also regulates negatively the innate antiviral immune response by blocking the type I IFN production pathway through direct association with RARRES3 and MAVS. Plays also a role in translation or delivery of incoming viral RNA to the translation apparatus.|
|Cellular Location||Cytoplasm. Preautophagosomal structure membrane; Peripheral membrane protein. Note=TECPR1 recruits the ATG12-ATG5 conjugate to the autolysosomal membrane.|
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Provided below are standard protocols that you may find useful for product applications.
Autophagy, the process of bulk degradation of cellular proteins thro µgh an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) thro µgh phosphorylation of autophagy protein APG1. ATG12, another member of the autophagy protein family, forms a conj µgate with ATG5; this conj µgate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conj µgate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. ATG12 has also been shown to interact with ATG10 in human embryonic kidney cells in the presence of ATG7. At least two isoforms of ATG12 are known to exist.
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