|Reactivity||Human, Mouse, Rat, Pig, Horse|
|Calculated MW||74867 Da|
|Application & Usage||Western blotting (0.5-4 µg/ml). However, the optimal conditions should be determined individually. The antibody recognizes ~75 kDa of Pak6 in Western blot analysis. Reactivity to other species has not been tested.|
|Other Names||Serine/threonine-protein kinase PAK 6; p21-activated kinase 6; PAK-6|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||PAK6 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Serine/threonine protein kinase that plays a role in the regulation of gene transcription. The kinase activity is induced by various effectors including AR or MAP2K6/MAPKK6. Phosphorylates the DNA-binding domain of androgen receptor/AR and thereby inhibits AR-mediated transcription. Inhibits also ESR1-mediated transcription. May play a role in cytoskeleton regulation by interacting with IQGAP1. May protect cells from apoptosis through phosphorylation of BAD (By similarity).|
|Cellular Location||Cytoplasm. Nucleus. Note=Cotranslocates into nucleus with AR in response to androgen induction.|
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Provided below are standard protocols that you may find useful for product applications.
The PAK (p21-activated kinase) family of serine/threonine kinases plays an important role in multiple cellular processes, including cytoskeletal reorganization, MAPK signaling, apoptotic signaling, etc. Several mechanisms that induce PAK activation have been reported. Binding of Rac/cdc42 to the CRIB (or PBD) domain at the N-terminal region of PAK causes autophosphorylation and conformational change of PAK. More recently identified members PAK4, PAK5 and PAK6 have lower sequence similarity with PAK1-3 in the regulatory N-terminal region.
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