|Reactivity||Human, Mouse, Rat|
|Calculated MW||126997 Da|
|Positive Control||3T3 cell lysate|
|Application & Usage||Western blot analysis (1-4 µg/ml). However, the optimal conditions should be determined individually. 3T3 cell lysate can be used as a positive control.|
|Other Names||Telomerase reverse transcriptase, HEST2, Telomerase catalytic subunit, Telomerase-associated protein 2, TP2, TEAP|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-TP2 polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||TP2 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Synonyms||EST2, TCS1, TRT|
|Function||Telomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA- dependent extension of 3'-chromosomal termini with the 6- nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis.|
|Cellular Location||Nucleus, nucleolus. Nucleus, nucleoplasm. Nucleus. Chromosome, telomere. Cytoplasm. Nucleus, PML body Note=Shuttling between nuclear and cytoplasm depends on cell cycle, phosphorylation states, transformation and DNA damage Diffuse localization in the nucleoplasm. Enriched in nucleoli of certain cell types. Translocated to the cytoplasm via nuclear pores in a CRM1/RAN-dependent manner involving oxidative stress- mediated phosphorylation at Tyr-707. Dephosphorylation at this site by SHP2 retains TERT in the nucleus. Translocated to the nucleus by phosphorylation by AKT|
|Tissue Location||Expressed at a high level in thymocyte subpopulations, at an intermediate level in tonsil T-lymphocytes, and at a low to undetectable level in peripheral blood T- lymphocytes.|
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Provided below are standard protocols that you may find useful for product applications.
Telomerase is an RNA-dependant DNA polymerase that catalyzes the addition of telemoric repeat, TTAGGG to chromosome ends. Telomerase is composed of an internal telomerase RNA template (TERC) and the enzyme, telomerase reverse transcriptase (TERT or TP2). Telomerase expression is usually repressed in postnatal somatic cells resulting in shortening of telomeres. Overexpression of telomerase may be implicated with oncogenesis.
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