|Reactivity||Human, Mouse, Rat|
|Calculated MW||69355 Da|
|Positive Control||Jurkat cell lysate, 3T3 cell lysate, rat kidney tissue lysate|
|Application & Usage||The antibody can be used for Western blotting (0.5–4 µg/ml).|
|Other Names||Cytosolic Phosphoenolpyruvate carboxykinase 1, PEPCK-C; Phosphoenolpyruvate carboxylase|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-PEPCK-C polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 0.01% thimerosal|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||PEPCK-C Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.|
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Provided below are standard protocols that you may find useful for product applications.
Phosphoenolpyruvate carboxykinase (PEPCK) is a key enzyme in stimulating glucose production. PEPCK catalyzes the conversion of oxaloacetate to phosphoenolpyruvate. There are two forms of PEPCK.,the cytosolic form also known as PEPCK-C and the mitochondrial form, or PEPCK-M.
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