|Reactivity||Human, Mouse, Rat|
|Calculated MW||120521 Da|
|Positive Control||Jurkat (Induced and Uninduced), Rat Kidney, 3T3 cell lysates|
|Application & Usage||The antibody can be used for Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Blocking peptide is available separately.|
|Other Names||Adipocyte determination- and differentiation-dependent factor 1, ADD1, Sterol regulatory element-binding transcription factor 1|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-SREBP1polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.01% thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||SREBP1 Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway (By similarity). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3') (PubMed:7739539). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA- 3') and to SRE-1 (5'-ATCACCCCAC-3').|
|Cellular Location||Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Cytoplasmic vesicle, COPII-coated vesicle membrane; Multi-pass membrane protein. Note=Moves from the endoplasmic reticulum to the Golgi in the absence of sterols|
|Tissue Location||Expressed predominantly in brown adipose tissue|
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Provided below are standard protocols that you may find useful for product applications.
SREBP (Sterol Regulatory Element Binding Protein) 1 and 2 are transcription factors th at regulates cholesterol homeostasis. SREBP proteins are attached to the endoplasmic reticulum and nuclear envelope. SREBP proteins undergo proteolytic cleavage in response to conditions of low cellular sterol. Upon activation of SREBP 1, a soluble NH2 cleavage fragment translocate to the nucleus and activates transcription of enzymes and other proteins required for cholesterol synthesis.
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