|Application & Usage||The antibody can be used for Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually.|
|Other Names||T7-tag, anti-T7-tag, T7-tag antibody, T7 antibody|
|Formulation||100 µg (0.5 mg/ml) affinity purified rabbit anti-T7-Tag polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 5 mM EDTA and 0.01 % thimerosal.|
|Handling||The antibody solution should be gently mixed before use.|
|Reconstitution & Storage||-20 °C|
|Precautions||T7 Epitope -Tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures.|
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Provided below are standard protocols that you may find useful for product applications.
The T7-tag is an 11 amino acid sequence encoded in the leader sequence of T7 bacteriophage gene10. T7 is used in many expression vectors including the pET system. Hence, the anti-T7-tag antibody is useful for studying expression of recombinant T7-tagged proteins.
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