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T7 Epitope -Tag Antibody
Rabbit Polyclonal Antibody
- SPECIFICATION
- CITATIONS
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- BACKGROUND
Application 
| WB |
|---|---|
| Reactivity | All Species |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Application & Usage | The antibody can be used for Western blot analysis (0.5-4 µg/ml). However, the optimal conditions should be determined individually. |
|---|---|
| Other Names | T7-tag, anti-T7-tag, T7-tag antibody, T7 antibody |
| Target/Specificity | T7-Tag |
| Antibody Form | Liquid |
| Appearance | Colorless liquid |
| Formulation | 100 µg (0.5 mg/ml) affinity purified rabbit anti-T7-Tag polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 5 mM EDTA and 0.01 % thimerosal. |
| Handling | The antibody solution should be gently mixed before use. |
| Reconstitution & Storage | -20 °C |
| Background Descriptions | |
| Precautions | T7 Epitope -Tag Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
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Provided below are standard protocols that you may find useful for product applications.
Background
The T7-tag is an 11 amino acid sequence encoded in the leader sequence of T7 bacteriophage gene10. T7 is used in many expression vectors including the pET system. Hence, the anti-T7-tag antibody is useful for studying expression of recombinant T7-tagged proteins.
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