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HA-Tag Antibody (Clone 12CA5[2])
Mouse Monoclonal Antibody
- SPECIFICATION
- CITATIONS
- PROTOCOLS
- BACKGROUND
Application 
| WB, IF, IP |
|---|---|
| Primary Accession | D5XIF4 |
| Other Accession | ADG21028 |
| Reactivity | All Species |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | Mouse IgG2b |
| Clone Names | 12CA5[2] |
| Application & Usage | Western blotting (0.5-4 µg/ml), immunoprecipitation (10-20 µg/ml), and immunocytochemistry (10-20 µg/ml). However, the optimal conditions should be determined individually. The antibody detects HA-tag fused to the C-terminus or N-terminus of targeted proteins in transfected mammalian cells. |
|---|---|
| Other Names | GRIN2A , hNR2A , NMDAR2A , NR2A |
| Target/Specificity | HA-Tag |
| Antibody Form | Liquid |
| Appearance | Colorless liquid |
| Formulation | 200 µg (1 mg/ml) anti-HA-Tag monoclonal antibody in phosphate buffered saline (PBS, pH 7.2), containing 50% glycerol, 1% BSA, 0.02% thimerosal. |
| Handling | The antibody solution should be gently mixed before use. |
| Reconstitution & Storage | -20 °C |
| Background Descriptions | |
| Precautions | HA-Tag Antibody (Clone 12CA5[2]) is for research use only and not for use in diagnostic or therapeutic procedures. |
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Provided below are standard protocols that you may find useful for product applications.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. The HA tag is derived from an epitope of the influenza hemagglutinin protein, which has been extensively used as a general epitope tag in expression vectors.
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